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KMID : 0861020130280020067
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Korea Journal of Herbology
2013 Volume.28 No. 2 p.67 ~ p.73
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The anti-inflammatory effect of Lithospermum Erythrorhizon on lipopolysaccharide - induced inflammatory response in RAW 264.7 cells
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최선복:Choi Sun-Bok
박경철:Park Kyoung-Chel/송호준:Song Ho-Joon/곽태신:Gwak Tae-Sin/이정현:Lee Jung-Hyun/이금산:Lee Guem-San/박성주:Park Sung-Joo/배기상:Bae Gi-Sang/조일주:Jo Il-Joo/서승희:Seo Seung-Hee/김동구:Kim Dong-Goo
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Abstract
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Objective ： Lithospermum Erythrorhizon (LE) has been used as an anti-bacterial and anti-inflammatory agent. However, it is unclear that LE aqueous extract could show the anti-inflammatory effects in RAW 264.7cells. The purpose of this study was to investigate the anti-inflammatory effect of aqueous extract from LE on lipopolysaccharide (LPS) - induced inflammatory response.
Methods ： To measure out the cytotoxicity of LE, we performed the MTT assay. To evaluate the anti-inflammatory
effects of LE, we examined the inflammatory mediators such as nitric oxide (NO), prostaglandin E2 (PGE2) and
pro-inflammatory cytokines (tumor necrosis factor (TNF)-α, interleukin, (IL)-1β and (IL)-6) on RAW 264.7 cells. We also examined molecular mechanisms such as mitogen-activated protein kinases (MAPKs) and nuclear factor-B (NF-κB) activation by western blot.
Results ： Aqueous Extract from LE itself did not have any cytotoxic effect in RAW 264.7 cells. Aqueous extract from LE inhibited LPS-induced productions of inflammatory mediators such as NO, PGE2, and pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 in RAW 264.7cells. In addition, LE inhibited the phosphorylation of p38 kinases (p38), c-Jun NH2-terminal kinase (JNK), and NF-κB activation in RAW 264.7 cells.
Conclusion ： LE down-regulated LPS-induced production of inflammatory mediators through the inhibition of p38, JNK and NF-κB activation. Taken together, these results could provide the evidence for the anti-inflammatory effects of LE. Therefore, LE may be a novel target in the management of inflammation and help to support a potential strategy for prevention and therapy of inflammatory diseases.
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KEYWORD
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Lipopolysaccharide (LPS), Lithospermum Erythrorhizon (LE), Inflammation, mitogen-activated protein kinases (MAPKs)
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